In this episode, Benjamin Callahan talks about some of the issues faced by

microbiologists when conducting amplicon sequencing and metagenomic studies. The two main themes are:

• Why one should probably avoid using OTUs (operational taxonomic units) and
  use exact sequence variants (also called amplicon sequence variants, or
  ASVs), and how DADA2 manages to deduce the exact sequences present in the
  sample.
• Why abundances inferred from community sequencing data are biased, and
  how we can model and correct this bias.

Links:

• Exact sequence variants should replace operational taxonomic units in marker-gene data analysis (Benjamin J Callahan, Paul J McMurdie & Susan P Holmes)
• DADA2: High-resolution sample inference from Illumina amplicon data (Benjamin J Callahan, Paul J McMurdie, Michael J Rosen, Andrew W Han, Amy Jo A Johnson & Susan P Holmes)
• In Nature, There Is Only Diversity (Michael R. McLaren, Benjamin J. Callahan)
• Consistent and correctable bias in metagenomic sequencing experiments (Michael R McLaren, Amy D Willis, Benjamin J Callahan)