Flow Cytometry has established as a critical technology for academic research and for the clinic. Approaching this technique requires understanding of the basic principles and expertise to set up, perform and analyze experiments to obtain reliable results. This newly launched ICGEB course aims to provide scientists with basic knowledge and hands-on experience to begin or improve their skills on flow-cytometry experiments, together with an overview of the most advanced techniques and biological questions that can be addressed using flow cytometry. Forum for discussion of practical issues and experimental design will be an integral part of the course to ensure immediate benefit for participants.